Where exactly the archaic hominin, Paranthropus robustus fits into the human evolutionary tree remains a subject of debate among palaeontologists. This species lived in southern Africa around 2 million years ago. They walked upright, indicating a shared ancestry with the Australopithecus and the later Homo genus. However, their comparatively small brains and massive jaws and teeth suggest a distinct evolutionary path, likely adapted for processing tough, fibrous plant material.
Determining their precise place in our evolutionary history would ideally require DNA analysis—but DNA does not survive long in the warm African climate. To overcome this limitation, a team of African and European researchers from the fields of molecular science, chemistry, and palaeoanthropology turned to a cutting-edge technique known as palaeoproteomics. By analysing proteins recovered from ancient tooth enamel, they were able to infer aspects of the underlying DNA, since the amino acid sequence in proteins is directly determined by the nucleotide sequence in DNA.
Their findings suggest that the story of early hominins is more complex than previously thought. There may have been more than one closely related species, with evidence of interbreeding or genetic divergence followed by remixing — patterns that would later come to characterise the tangled branches of the hominin family tree.
The research team included three postdoctoral scientists from the University of Copenhagen — Palesa P. Madupe, Claire Koenig, and Ioannis Patramanis — who have written about their work and its significance in the open-access magazine The Conversation.
Their findings are also published in Science.
Their article in The Conversation is reproduced here under a Creative Commons licence, reformatted for stylistic consistency:
New clues from 2 million-year-old tooth enamel tell us more about an ancient relative of humans
Proteins were taken from the enamel of this Paranthropus robustus’ tooth. Courtesy Dr. Bernhard Zipfel, with permission from Dr. Lazarus Kgasi, junior curator of Plio-Pleistocene Paleontology at Ditsong National Museum of Natural History in Pretoria, South Africa.
Palesa P. Madupe, University of Copenhagen; Claire Koenig, University of Copenhagen, and Ioannis Patramanis, University of Copenhagen
For nearly a century, scientists have been puzzling over fossils from a strange and robust-looking distant relative of early humans: Paranthropus robustus. It walked upright, and was built for heavy chewing with relatively massive jaws, and huge teeth with thick dental enamel. It’s thought to have lived between 2.25 million and 1.7 million years ago.
Humans today have a diverse array of hominin distant relatives and ancestors from millions of years ago. The South African fossil record ranges from early hominins such as Australopithecus prometheus, A. africanus (Taung child), A. sediba and P. robustus, to early members of the genus Homo (H. erectus/ergaster, H. habilis), to later hominins such as H. naledi and Homo sapiens (humans).
Fossils show how these early relatives evolved from as far back as A. africanus, 3.67 million years ago. They also document milestones in evolution, including the transition to walking on two legs, tool making and increased brain development. Ultimately, our species – Homo sapiens – appeared in South Africa 153,000 years ago.
Fossils of P. robustus were first discovered in South Africa in 1938. But crucial questions remained. How much variation was there within the species? Were the size differences related to sex, or did they reflect the presence of multiple species? How was P. robustus related to the other hominins and early Homo? And what, genetically, made it distinct?
Study co-leads Dr Palesa Madupe, Dr Claire Koenig and Dr Ioannis Patramanis with a replica of Paranthropus robustus.
Victor Yan Kin Lee
We experienced a breakthrough when we decided to use palaeoproteomics – the analysis of ancient proteins. We extracted these from the enamel of the 2-million-year-old teeth of four P. robustus fossils from Swartkrans Cave in South Africa’s Cradle of Humankind.
Luckily, proteins that are millions of years old preserve well because they stick to teeth and bones and are not affected by the warm weather. One of these proteins tells us the biological sex of the fossils. This is how we found that two of the individuals were male and two were female.
These findings open a new window into human evolution – one that could reshape how we interpret diversity in our early ancestors by providing some of the oldest human genetic data from Africa. From there, we can understand more about the relationships between the individuals and potentially even whether the fossils come from different species.
More than one kind of Paranthropus?
The protein sequences also revealed other subtle but potentially significant genetic differences. One standout difference was found in a gene which makes enamelin, a critical enamel-forming protein. We found that two of the individuals shared an amino acid with modern and early humans, chimpanzees and gorillas. The other two had an amino acid that among African great apes is, so far, unique to Paranthropus.
What’s even more interesting is that one of the individuals had both the distinct amino acids. This is the first documented time we can show heterozygosity (a state of having two different versions of a gene) in proteins that are 2 million years old.
When studying proteins, specific mutations are thought to indicate different species. We were quite surprised to discover that what we initially thought was a mutation unique to Paranthropus robustus was actually variable within that group – some individuals had it while others did not. Again, this was the first time anyone had observed a protein mutation in ancient proteins (these mutations are usually observed in ancient DNA).
We realised that instead of seeing a single, variable species, we might be looking at a complex evolutionary puzzle of individuals with different ancestries. This shows that combining analyses of morphology (the study of the form and structure of organisms) and the study of ancient proteins, we can create a clearer evolutionary picture of the relationships among these early hominin individuals.
However, to confirm that P. robustus fossils have different ancestry, we will need to take samples of tooth enamel protein from more of their teeth. To do this, we plan to sustainably sample more P. robustus from other sites in South Africa where they’ve been found.
Preserving Africa’s fossil heritage
Our team was careful to balance scientific innovation with the need to protect irreplaceable heritage. Fossils were sampled minimally, and all work followed South African regulations. We also involved local laboratories in the analysis. Many of the authors were from the African continent. They were instrumental in guiding the research agenda and approach from the early stages of the project.
Doing this kind of high-end science on African fossils in Africa is an important step towards transformation and decolonisation of palaeontology. It builds local capacity and ensures that discoveries benefit the regions from which the fossils come.
By combining data on molecules and morphology, our study offers a blueprint for future research – one that could clarify whether early hominins were more or less diverse than we’ve known.
For now, the Paranthropus puzzle just got a little more complex – and a lot more exciting. As palaeoproteomic techniques improve and more fossils are analysed, we can expect more surprises from our ancient relatives.
(Jesper V. Olsen, Rebecca R. Ackermann and Enrico Cappellini were also the principal investigators on this project.)
Palesa P. Madupe, Postdoctoral Researcher, University of Copenhagen; Claire Koenig, Post doc researcher, University of Copenhagen, and Ioannis Patramanis, Postdoctoral Researcher, University of Copenhagen
This article is republished from The Conversation under a Creative Commons license. Read the original article.
Abstract
Paranthropus robustus is a morphologically well-documented Early Pleistocene hominin species from southern Africa with no genetic evidence reported so far. In this work, we describe the mass spectrometric sequencing of enamel peptides from four ~2 million–year-old dental specimens attributed morphologically to P. robustus from the site of Swartkrans in South Africa. The identification of AMELY-specific peptides enabled us to assign two specimens to male individuals, whereas semiquantitative mass spectrometric data analysis attributed the other two to females. A single amino acid polymorphism and the enamel-dentine junction shape variation indicated potential subgroups present within southern African Paranthropus. This study demonstrates how palaeoproteomics can help distinguish sexual dimorphism from other sources of variation in African Early Pleistocene hominins.
Palesa P. Madupe et al.
Enamel proteins reveal biological sex and genetic variability in southern African Paranthropus.
Science 388, 969-973 (2025).DOI:10.1126/science.adt9539
© 2025 the American Association for the Advancement of Science.
Reprinted under the terms of s60 of the Copyright, Designs and Patents Act 1988.
This kind of research stands in stark contrast to the claims of young-Earth creationists, who typically base their understanding of human origins on literal interpretations of ancient texts such as the Book of Genesis. According to these beliefs, all humans descend from a single pair—Adam and Eve—created fully formed just a few thousand years ago. The idea that there could have been multiple hominin species, with complex evolutionary relationships and possible interbreeding over millions of years, is entirely incompatible with such a view.
What palaeoproteomics reveals is a deep and nuanced evolutionary history, one written not in mythology but in the very molecules preserved in ancient remains. These protein sequences offer hard, testable evidence of ancestry and divergence, confirming that humans are the product of a long and branching evolutionary process. Unlike ancient scriptures, these findings are not based on oral tradition, religious authority or metaphor, but on observable biochemical relationships that can be verified and reproduced in laboratories around the world.
Creationist accounts, by contrast, are rooted in Bronze Age cosmologies that lacked even the most basic understanding of biology, geology, or deep time. They reflect the worldview of early agricultural societies trying to make sense of the world with no knowledge of genetics, fossilisation, or molecular science. To treat such texts as literal science is to ignore the overwhelming body of empirical evidence that shows humanity's past to be far richer, longer, and more complex than any ancient myth could have imagined.
What makes this research particularly powerful is not just what it tells us about Paranthropus robustus, but what it demonstrates about the tools of modern science. Techniques like proteomics are allowing us to uncover new layers of the human story—layers completely inaccessible to those who reject evolution on theological grounds. As science continues to advance, the gap between evidence-based understanding and myth-based belief only widens.
Advertisement
What Makes You So Special? From The Big Bang To You
How did you come to be here, now? This books takes you from the Big Bang to the evolution of modern humans and the history of human cultures, showing that science is an adventure of discovery and a source of limitless wonder, giving us richer and more rewarding appreciation of the phenomenal privilege of merely being alive and able to begin to understand it all.
Ten Reasons To Lose Faith: And Why You Are Better Off Without It
This book explains why faith is a fallacy and serves no useful purpose other than providing an excuse for pretending to know things that are unknown. It also explains how losing faith liberates former sufferers from fear, delusion and the control of others, freeing them to see the world in a different light, to recognise the injustices that religions cause and to accept people for who they are, not which group they happened to be born in. A society based on atheist, Humanist principles would be a less divided, more inclusive, more peaceful society and one more appreciative of the one opportunity that life gives us to enjoy and wonder at the world we live in.
Amazon
Amazon
Amazon
Amazon
Amazon
Amazon
Amazon
Amazon
All titles available in paperback, hardcover, ebook for Kindle and audio format.
Prices correct at time of publication. for current prices.
No comments :
Post a Comment
Obscene, threatening or obnoxious messages, preaching, abuse and spam will be removed, as will anything by known Internet trolls and stalkers, by known sock-puppet accounts and anything not connected with the post,
A claim made without evidence can be dismissed without evidence. Remember: your opinion is not an established fact unless corroborated.