The findings support a stepwise model for how the genetic program that builds vertebrate paired fins and limbs may have evolved. An ancestral Hox9–10 program may first have helped establish posterior appendages, later expanded into a broader Hox patterning system, and eventually been redeployed in anterior paired appendages.
Image after H. Nakazawa et al., (2026) CC BY-NC-ND.
Creationists who insist that fish and terrestrial vertebrates are separately created “kinds” have another piece of evidence to explain away. Beneath the obvious differences between a fish’s pelvic fins and an amphibian’s hindlimbs lies a shared developmental inheritance: related genes performing an essential early role in establishing the cells from which those structures develop. Evolutionary relationships are recorded not only in fossils and adult anatomy, but also in the processes that build living bodies.
This is illustrated by research led by scientists at Saitama University, Japan, published in Proceedings of the National Academy of Sciences (PNAS). The researchers investigated Hox genes, regulatory genes involved in organising the developing body. Their experiments reveal a shared requirement for members of the Hox9–10 groups at the beginning of pelvic-fin and hindlimb development.
In zebrafish, disrupting five of these genes together eliminated the detectable populations of pelvic-fin progenitor cells—the cells that would normally give rise to the developing fins—and the pelvic fins failed to form. In Iberian ribbed newts, disrupting seven related genes likewise impaired the establishment of hindlimb progenitors. These results support the inference that this developmental function was already present in the common ancestor of ray-finned and lobe-finned vertebrates, before the evolutionary separation of the lineages represented by zebrafish and newts.
The significance extends beyond the discovery that different animals use related genes. The findings help connect the genetic programme that organises the rear of the body with the programme that initiates its paired hind appendages. They are consistent with an evolutionary model in which an ancestral programme was recruited, expanded and redeployed as the developmental machinery of paired fins and limbs evolved. The experiments do not directly reconstruct that entire historical sequence, but they provide functional evidence with which to assess it.
For intelligent design creationism, the familiar difficulty is that evolutionary innovation need not involve inventing every component afresh. Existing genes and developmental relationships can acquire additional roles as evolution modifies inherited systems. Calling those systems “designed” supplies no comparable account of when, where or how the changes occurred, nor does it improve the experimental explanation.





































